Regulatory T Cell Staining Kit
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The protocol recommends using 1 x 10 6 pbmcs but it can be adjusted for staining other cells e g.
Regulatory t cell staining kit. One kit is sufficient for staining 25 samples. Immunoblotting with fjk 16s antibody has mapped the epitope to amino acids 75 125 of the mouse foxp3 protein. Foxp3 is a 49 55 kda protein and a. 88 8115 ebioscience mouse regulatory t cell staining kit 3 fjk 16s for research use only.
The pch101 monoclonal antibody reacts with the amino terminus of human foxp3 also known as forkhead box p3 scurfin and jm2. The treg detection kit cd4 cd25 foxp3 includes all reagents necessary for cell surface staining of cd4 and cd25 and intracellular staining of foxp3 with a special foxp3 staining buffer set. Mouse regulatory t cell staining kit 2 kit from thermo fisher scientific. Constitutive high expression of foxp3 mrna has been shown in cd4 cd25 regulatory t cells treg cells and ectopic expression of foxp3 in cd4 cd25 cells imparts a treg phenotype in these cells.
Immunoblotting with fjk 16s antibody has mapped the epitope to amino acids 75 125 of the mouse foxp3 protein. Foxp3 staining protocol for mouse tissues introduction. Cross reactivity of this antibody to other proteins has not been determined. Ebioscience 88 8115 40 product information.
In our experience the human regulatory t cell staining kit from ebioscience is a reliable and straightforward tool for detection of cd4 cd25 treg cells expressing foxp3. Constitutive high expression of foxp3 mrna has been shown in cd4 cd25 regulatory t cells treg cells and ectopic expression of foxp3 in cd4 cd25 cells imparts a treg phenotype in these cells. This human regulatory t cell whole blood staining kit contains the buffers and monoclonal antibody necessary to successfully stain and identify foxp3 cells in whole blood samples. Expanded effectors or tils.
Not for use in diagnostic procedures. Distributor quantity price mouse regulatory t cell staining kit 3 kit. Description the fjk 16s antibody reacts with mouse rat foxp3 also known as forkhead box p3 scurfin and jm2. The following protocol allows the simultaneous analysis of cell surface molecules and intracellular antigens including nuclear antigens.
The kit also includes an optimized protocol.
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